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Petite H(1), Frei V, Huc A, Herbage D. Author information: (1)Coletica, Lyon, France. Cross-linking of collagen-based biomaterials increases their strength and. By DANIEL HERBAGE, JOSETTE BOUILLET and JEAN-CLAUDE Solubilization of collagen from bovine articular cartilage with pepsin. The formation of new bonds between the denatured collagen chains and the proteoglycans can explain the D. Herbage, A. Huc, D. Chabrand, M.C. Chapuy.

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Application of Collagen Scaffold in Tissue Engineering: Citations Publications citing this paper. Treatment of native bovine pericardium with 0.

Use of diphenylphosphorylazide for cross-linking collagen-based biomaterials.

Topics Discussed in This Paper. Cross-linking of collagen-based biomaterials increases their strength and persistence in vivo. Showing of 71 extracted citations.

Chevallay and Dr D. In the 10 7 cells-seeded sponges, mineral deposition, observed with unseeded sponges, was significantly collayen 2- to 3-fold. Aluminum Oxide Search for additional papers on this topic. Monday, October 29, – 9: Specific cartilage collagens types II and XI were deposited in the matrix throughout the culture and collgen of type I collagen were noticed only in the culture medium after weeks and 4 weeks in the case of 10 6 and 10 7 cells-seeded sponges, respectively.

By clicking accept or continuing to use the site, you agree to the terms outlined in our Privacy Policy hsrbage, Terms of Serviceand Dataset Herbagf. Both supports were always present during the study and a partial decrease in size and weight was detected only with control sponges, both seeded and unseeded.

From This Paper Figures, tables, and topics from this paper. Collagen-based biomaterials in the form of sponges bovine type I collagen, both native and cross-linked by treatment with diphenylphosphorylazide, hdrbage control and DPPA sponges respectively were tested as three-dimensional scaffolds to support chondrocyte proliferation with maintenance of the phenotype in order to form neocartilage.


Mineral deposition Glycosaminoglycan deposition Articular cartilage repair Collagen-based biomaterial Collagen synthesis. BonassarCharles A. Native and DPPA cross-linked collagen sponges seeded with fetal bovine epiphyseal chondrocytes used for cartilage tissue engineering.

ChevallayDr D.

Collagen References

Allogeneic Mesenchymal Stem Cells and Biomaterials: Vacanti Journal of cellular biochemistry. These in vitro results indicate that both collagen matrices can support collagwn development of tissue engineered cartilage. Collagen-based biomaterials as 3D scaffold for cell cultures: Cross-linking of dermal sheep collagen using a water-soluble carbodiimide.

Mouse fibroblasts in long-term culture within collagen three-dimensional scaffolds: In this report, we propose a simpler, faster way to prepare acyl azide groups and to cross-link collagen-based biomaterials, using diphenylphosphorylazide DPPA as a reagent.

Immunologically active biomaterials for cancer therapy. Have you forgotten your login? In order to validate and quantitate coklagen extent of reaction, the degree of cross-linking was determined by the measure of the free primary amino group content of the samples.

Ham A Benghuzzi Biomedical sciences instrumentation Metals Organ Ceramics Chitosan polylactic acid-polyglycolic acid copolymer. Gelatine-Based Biomaterials and Their Biocompatibility: Cytological evaluation of capsular tissue surrounding TCPL implant in adult rats.

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Methods for the treatment hrrbage collagenous tissues for bioprostheses. Howard The Journal of biological chemistry Similar decreases in free primary amino group content and increases in thermal stability were obtained for collagen films treated with DPPA, glutaraldehyde, or hydrazine.


Files produced by the author s. Cell proliferation was only noted in the 10 6 cells-seeded sponges 4-fold increase after 4 weeks of culture. Collagen as matrix for neo-organ formation by gene-transfected fibroblasts. Showing of 84 references. Gelatinase A activation is regulated by the organization of the polymerized actin cytoskeleton. Glycosaminoglycans accumulated collaen the matrix, up to 1.

With the rapid development in tissue engineering, these different biomaterials have been used as three-dimensional scaffolds and cell transplant devices. Anne-Marie Freyria 1 AuthorId: Skip to search form Skip to main content.

References Publications referenced by this paper. After determining the optimal conditions of cross-linking with DPPA, we compared the efficiency of this protocol with that using hydrazine and with the classical glutaraldehyde treatment.

BiomaterialsElsevier,22, pp. AliDavid J Mooney Current topics in microbiology and immunology Wednesday, December 13, – 2: The Perfect Match for Cardiac Repair?

DPPA-treated pericardium had a collgaen to collagenase digestion similar to that of glutaraldehyde- or hydrazine-treated pericardium. These collagdn were corroborated by resistance to bacterial collagenase digestion: RosenthalGabriele J Koehler Anticancer research Daniel Herbage 1 AuthorId: Development and characterisation of a three-dimensional in vitro hepatic fish model to investigate xenobiotic metabolism Matthew G Baron Recently, we described an efficient cross-linking process via the formation of acyl azide groups on methylated carboxyl groups of collagen using hydrazine and nitrous acid referred to here as the hydrazine method.